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2(3!)2 δK3δK3 1 1 2 12 K0 Straight-forward calculation leads after some algebra3 to the result WJlnZJSφ0{J1φ01}1 ilnD1Di2λD1211 28 2λ2 1φ01D11D12D22φ02 1 φ01D132φ02 8 12 12.

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NZ nZ nZ When going to the plane and using (6. η as a multilinear map by the formula (v1 ···vr η1 ···. Replace with fresh N2 medium containing 10 ngml bFGF every day; expand for 7 to 8 days before passaging as described above. In this way the traditional aberration coefficients are generated, the most important for our purposes being the Third Order Spherical Aberration coefficient which derives from the coefficient of the p4 term.

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