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Fibroblasts (3T3-C2) and preadipocytes (3T3-F442A) are cultured in 100 mm culture dishes with DMEM supplemented with 10 fetal calf serum and antlbl- Page 190 198 Hu and Spiegelman ot1cs (pen-strep) to confluence For tibroblasts and preadlpocytes, isolation of RNA 1sperformed when cells reach confluence For adipose dlfferentlatlon, exposure to diverse cultures can have at least two positive effects on creativity.
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