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(1999) When is enough enough. Myogenin 5AGCTCCCTCAACCAGGAGGA3 and 5GGGCTCTCTGGACTCCA TCT3, annealing temperature 60C, size of the cDNA 500 bp, size of genomic DNA band 1500 bp Hypoxanthine Phosphoribosyltransferase (HPRT, as a standard to balance the free of RNA and cDNA used) 50GCTGGTGAAAAGGACCTCT30 and 50CACAGGACTAGAACACCT GC30, annealing temperature Rs, size of the cDNA 249 bp, size of genomic DNA band 1100 bp Page 336 19 ES CELLS INTO HEPATOCYTES 277 Acknowledgment This work was supported by the Association pour la Recherche Contre le Cancer (grant no.
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