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With elements of similar chemical properties arranged in columns. 8 LINEAR CHAIN WITH TWO MASSES PER UNIT CELL To see the effects of introducing t ax degrees of freedom in the crystal cell, we next consider a slightly more complicated one dimensional crystal with two inequivalent atoms in ench repeated unit.
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Page 85 84 CHAPTER 5 Hpa I 2777 ori 264 377 T7 promoter Cla I 3018 tac promoter purification tag sequence 1-386 EcoR I 3277 Pst I 3316 Sal I 52 BstX I Sac Tax Nde I 532 Nru I Hind III Pvu II BamH I Acc65 I Kpn I EcoR V Bgl II Sma I Not I SP6 PinPoint Xa-1 Vector (3351bp) Ampr Factor Xa site 379-390 389 392 400 404 410 414 418 422 430 435 452 TM Fsp I 1483 Xmn I 1106 Pvu I 1337 Sca I 1225 It is possible to engineer proteins such that they are transported through the outer membrane and are secreted into the growth medium.
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