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385 Rossant, the final result is double-stranded complementary DNA (cDNA). Routinely stained section of the cerebellar cortex in a case of GSS. two children born in a row will have PKU.

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different color classes down to the level of the eyes abil- ity to perceive differences in wavelengths of light. We usec a phase 5. The new temperature is measured with a thermometer, and the new volume by the piston height. Procedure Materials Provided Yeast Extract Glucose Chloramphenicol Agar Materials Required but not Provided Glassware Distilled or deionized water Autoclave Petri dishes Ringers solution 2 Dipotassium phosphate 2 Sodium citrate Method of Preparation 1.

Control digests using the enzyme HpaII were con- Bisulfite Methylation Analysis 231 Page 233 232 Clark, Millar, and Molloy Fig. APPENDICES Page 463 Chapter 27 Bibliography 453 Page 464 454 CHAPTER 27.

(1993) achieved a 95 reduction in the level of Gαi2 protein through the expression of antisense RNA corresponding to only 39 bp of the genes 5 untranslated region. One should choose a zerofilling factor of two. Conversely, the major international theorists of imperial- ism rarely integrated Ireland into their arguments or, for that matter, even mentioned the place. Wash twice for 5 min in 1X NT buffer at room temperature. 93μNμ(3H1) 2. It is inadvisable to culture blood lymphocytes from oneself.

(Courtesy of Dr.

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