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The following primer sequences were used for the real time PCR amplification Cbfa1Primer 1 TGC TTC ATT CGC CTC ACA AA, Primer 2 TGC TGT CCT CCT GGA GAA AGT T, Probe AAC CAC AGA ACC ACA AGT GCG GTG C; Type I Collagen Primer 1 CTG CCG ATG TCG CTA TCC A, Primer 2 AGG TGA TGT TCT GGG AGG CC, Probe TCC TGC GCC TGA TGT CCA CCG, Osteocalcin primer 1 CGG CCC TGA GTC TGA CAA A, Primer Forex pitbull trading GCC GGA Forex pitbull trading TGT TCA CTA CTA CCT T.

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Suspend the medium in 1 liter distilled or deionized water Tryptic Soy Broth - 30 grams; Tryptic Soy Broth wo Dextrose - 27.

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