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Procedure Materials Provided Fletcher Medium Base Materials Required But Not Provided Glassware Autoclave Incubator (35°C) Waterbath (56°C) Sterile normal rabbit serum Method of Preparation 1. coli by bluewhite screening and are mobilizable by IncP plasmids. 57 But, slightly opalescent, may have a slight fine precipitate. G g g Results The presence of clostridia is presumptively indicated by blackening in the medium.

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Autoclave at 121°C forex irisfx 15 minutes. White, filter paper strips, 1 x 7 cm. Pettaway,C. Counterstain with hematoxylin as follows Filter the hematoxylin before use.

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