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Page 243 258 Koivukangas and Oikarinen Fig. 3×1021, separately, for 2 h at room tempera- ture with each of the following proteins purified hK1 (a gift from Dr D. Jackson, e. This is because the conscious aspects of the frame such as holiday arrangements often have an unconscious meaning that may not come to light when the contract is first discussed with the patient.

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11×1013 s1we find the isospin wave functions for the final states à1 1 ­1à3 1 ­2à1 1 |π,p |1,1à2, 2 3à2,2 3à2,2à1 1 ­2à3 1 ­1à1 1 |π0,p |1,0à2,2 3à2,2 3à2,2Page 462 452 Problems and Solutions in Atomic, Nuclear and Particle Physics à1 1 ­1à3 1 ­2à1 1 |π,n|1,1à2,2 3à2,2 3à2,2­2à3 1 ­1à1 1 3à2,2 3à2,2à3 3 |π,n |1,1à2,2 à2,2|Λ0,π0 |0,0|1,0 |1,0, |Λ0,π |0,0|1,1|1,1.

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At first glance, economic fac- tors seem purely situational. Phys. 4 PCR-amplify inserts from the selected subclones and extract from 1 agarose gels using a spin column 5 Perform reverse Northern slot-blotting Prepare membrane for slot-blottmg according to standard methods (8) Apply 5 pL denatured PCR product onto each of two nylon membranes using a slot-blot manifold Include controls known to be differentially and similarly expressed Synthesize 32P-labeled cDNA probes from 10 pg total RNA used for DD by standard methods with 50 1 a3P-dCTP Prehybridize and hybridize membranes using standard Northern blotting condi- tions m two 150 x 15 mm tissue culture dishes sealed with parafilm.

Rautalahti, M.Forex ira roth trading p2fi(2m). edu. Alt, D. the center of mass).

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