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However, it is still impor- tant for cDNA cloning (see p. Page 158 7 THE POSSIBILITY OF IN VITRO ORGANOGENESIS 111 Cell line ST2 OP9 ES cells EF()ES cells Condition 100 confluency 70 confluency 70 forex gmt open 70 confluency Medium for freezing FBS10DMSO MEM20FBS10DMSO ESM10DMSO EF()ESM10DMSO Optimal density 100-mm dish2 tubes 100-mm dish2 tubes 5106 cellstube 5106 cellstube TABLE III RECOMMENDED CONDITIONS FOR FREEZING CELLS 2.
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