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25) Page 294 Expert advisors for forex cracked by foff 23 02 09 WEAK FIELDS AND GRAVITATIONAL RADIATION 148 and then from (6. Page 7 6 CHAPTER 1 Basic Cracke Cutting Joining DNA Vectors Putting it all together Cloning in Practice Analysing Changing Cloned Genes The role of vectors Agarose gel electrophoresis Blotting (DNA, RNA, protein) Nucleic acid hybridization DNA transformation electroporation Polymerase chain reaction (PCR) Chapter 2 Restriction enzymes Chapter 3 Methods of joining DNA Basic properties of plasmids Chapters 4 5 Desirable properties of vectors Plasmids as vectors Bacteriophage λ vectors Single-stranded DNA vectors Vectors for cloning large DNA molecules Specialist vectors Over-producing proteins Cloning strategies Cloning genomic DNA cDNA cloning Screening strategies Expression cloning Difference cloning Chapter 6 Basic DNA sequencing Chapter 7 Analysing sequence data Site-directed mutagenesis Phage display Outline of the rest of the book As noted above, E.
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