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6gin20mLofH2OandadjusttopH 5. 4, for 2 h at room temperature (see Notes 2 and 3). ENTROPY ACCOUNTING AND APPLICATIONS 164 and entropy accounting Eq. We have used two approaches to address this problem. When she heard screams, Brokre ran to ask her associates to stop but was too late.
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The region above F2 is called the topside ionosphere. L of 5X first-strand buffer, 2 of DTT, 2 uL of dNTP mix, 2 of oligo(dT) primer, and 7 Ils, of DEPC water Heat the mix for 5 mm at 65°C and incubate for 10 mm at 37°C for primer annealmg Add 1 pL (200 U) of M-MLV Reverse Tran- scriptase.
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