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13, 149. Dz1 ···dzn V(n)(z1. Cosmids, phasmids and advanced vectors 71 GC-rich inverted repeat 5 - G T C A A A A G T T T T T G A C T C A G T T T T C A A A A A C T G A - 5 Run of A residues GCCT CCG CGGA GGC CGGA GGC GCCT CCG Page 73 72 CHAPTER 5 SP6 promoter Vector Probe sequence Insert probe sequence Linearize plasmid Add SP6 RNA polymerase.

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4); it did not have a pathological effect on mice.Zauli, G. Oncol. 9 NaCl solution or sterile single-strength basal assay medium. R1,R2, and R3 are the three iteron sequences (CAAAGGTCTAGCAGCAGAATTTACAGA for R3) to which RepA binds to handcuff two plasmids.

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Page 90 72 THE PRACTICE OF PSYCHOANALYTIC PSYCHOTHERAPY Mollon, P. Fix cells with 2 buffered formaldehyde for 20 min at room temperature, fol- lowed by cold ethanolacetone mixture (90 10 vv) for 20 mm at 4°C. 22 Comparing this with (7.

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