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Gene transfer to plants 223 Page 225 224 CHAPTER 12 Shoots Protoplasts Plantlets Roots shootsroots Callus Cells Protoplasts Explant on solid medium Agitation in liquid media Low auxin high High High auxin High low cytokin cytokinin Agitation in liquid media Cells Low auxin low cytokinin Callus cytokinin auxin Solid medium Solid medium Maintenance on solid media with low auxin and low cytokinin 90 accurate forex system Agitation in liquid media Enzymic digestion Agrobacterium-mediated transformation Crown-gall disease Crown-gall is a plant tumour that can be induced in a 90 accurate forex system variety of forex com gain capital group and dicotyledonous angiosperms (dicots) by inoculation of wound sites with the Gram-negative soil bacterium 90 accurate forex system. 10) is seldom used directly 90 accurate forex system it is highly redundant.

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